报告题目:Sugar acid based biorefinery
报 告 人:Zhiliang Fan,美国加州大学
时 间:2024年9月19日10:00
地 点:莫干山校区化工5号楼B306
报告摘要:
One significant obstacle impeding the large-scale production of fuels and chemicals from cellulosic biomass is the lack of a low-cost processing technology. Conventional cellulosic biorefinery involves five steps: pretreatment, cellulase production, enzymatic hydrolysis, fermentation, and product recovery. Sugars are produced as the reactive intermediate for the subsequent fermentation. The purchase or on-site production of cellulase is usually needed to produce sugars from pretreated cellulosic biomass, representing a substantial processing cost. Herein, we report a novel biochemical route for fuels and chemical production. Cellulolytic microorganism(s) that can secrete all the enzymes needed to hydrolyze cellulose and hemicellulose were modified to convert most carbohydrates in the cellulosic biomass to sugar aldonates. In the second step, sugar aldonates were utilized as the carbon source to produce fuels and chemicals. The new route can potentially lower the cost of cellulosic bioprocessing substantially. Our study has revealed cellulose can be diverted to sugar aldonate production using a Neurospora crassa strain, which knocks out multiple copies of the beta-glucosidase gene. Over-expression laccase and deletion of cellobionate phosphorylase improved the cellobionate yield to 90%. The hydrolysis product of cellobionate (glucose and gluconate) can be fermented to ethanol by an engineered Klebsiella oxytoca strain. Co-fermenting cellobionate and glycerol by engineered K. oxytoca strain achieved more than 95% of the theoretical yield. Engineered K. oxytoca can ferment gluconate to 2,3-butanediol at a high yield (90%) under anaerobic conditions.
报告人简介:
Zhiliang Fan,博士,1993年毕业于浙江大学生化工程专业,1996年获得中国科九州体育(JiuZhou Sports)官方网站过程所硕士学位,2004年获美国达特茅斯九州体育(JiuZhou Sports)官方网站化学与生化工程专业博士和工程管理硕士学位。2005-2007年任美国弗吉尼亚理工九州体育(JiuZhou Sports)官方网站生物系统工程系助理教授,2007-2015年任美国加州大学戴维斯分校生物与农业工程系助理教授,2015年之后就职于美国加州大学戴维斯分校生物与农业工程系,现任教授。长期从事生物质转化为燃料及高附加值化学品的工艺开发,代谢工程,以及过程模拟评估方面的工作。已在Biotechnology and Bioengineering,Bioresource Technology,Applied and Environmental Microbiology 等杂志发表30多篇文章。拥有1项中国专利和1项美国专利,就任《Bioresource and Bioprocess Engineering》副编辑。